You're One In A Googol: Optimizing Genes For Protein Expression

Heterologous protein expression is fundamental to biotechnology. Still, there has been little systematic analysis of the relationship between gene design and expression success.


Advances in synthetic biology now provide the impetus to transform protein expression folklore into design principles, so that DNA sequences may be designed to express any protein at functional levels in any system.


In this review we offer a brief survey of the literature, outline the major challenges in interpreting existing data and constructing robust design algorithms, and propose a way to proceed toward the goal of rational sequence engineering.


Journal of the Royal Society Interface


Journal of the Royal Society Interface is the Society's cross-disciplinary publication promoting research at the interface between the physical and life sciences. It offers rapidity, visibility and high-quality peer review and is ranked fifth in JCR's multidisciplinary category. The journal also incorporates Interface Focus, a peer-reviewed, themed supplement, each issue of which concentrates on a specific cross-disciplinary subject.


Journal of the Royal Society Interface